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SNCA Knockout Cell Lines Drive Parkinson’s Disease Research
Novel tools for studying the effects of varying alpha-synuclein expression levels
Available at ATCC

SNCA triplication = original iPSC that went through the editing process, Iowa kindred,  hg19, Chr.4: 89,33,7274–91,04,7192 (Zafar et al., 2018)
4 functional SNCA gene copies
SNCA3x_0KO_C1
SNCA3X_0KO_C2

Knockout of 1 SNCA allele = SNCA duplication/
3 functional SNCA gene copies
SNCA3x_1KO_C1
SNCA3X_1KO_C2

Knockout of 2 SNCA alleles = Normal copy number
2 functional SNCA gene copies
SNCA3x_2KO_C1
SNCA3x_2KO_C2

Knockout of 3 SNCA alleles =
1 functional SNCA gene copy
SNCA3x_3KO_C1
SNCA3x_3KO_C2
SNCA3x_3KO_C3

Knockout of 4 SNCA alleles = No functional SNCA gene copies
SNCA3X_4KO_C1
SNCA3x_4KO_C2
SNCA3X_4KO_C3

These cell lines were supported by the Michael J. Fox Foundation

Reference:
Lab Resource: Genetically-Modified Multiple Cell Lines
Isogenic human SNCA gene dosage induced pluripotent stem cells to model Parkinson’s disease